qPCR Users:Transition to QuantStudio60-Hudson Medical Genomics Platform, seminar on 23 March & 9 April

The qPCR 7900HT systems have serviced us well since 2006, however, following notification from Thermofisher Scientific that they intend to phase out service support for the 7900HT instruments this year, we will be replacing these system with new Quantstudio 6 real time instruments. Quantstudio 6 instruments will support 384 well format runs and whilst the software is similar, training will be required prior to booking the system or commencing use.

All current users of the qPCR 7900HT system and new Quantstudio 6 users are required to please attend one of the following seminars presented by Life Technologies (attendance will be recorded as your induction to the new system):

10am to 11am – Seminar room 1 - TRF level 2

Friday March 23^rd  OR  Monday – April 9^th

Please note, transisition will involve:

·         7900HT Instruments will remain in use and will be able to be booked until June 30^th 2018

·         All users are asked to backup their own data and templates from the 7900HT computers by June 30^th 2018

·         Quantsudio 6 instruments will be available for booking via ARIN from March 26^th 2018 (charges are identical for use)

·         The Facility is undertaking internal verification of the Quantstudio 6 systems and we encourage all users to perform their own verification as required for research

·         The new process for data retrieval will be covered during the seminars

Resources:

·         Key applications: Quantstudio6 capabilities are similar to the 7900HT system in key applications including Gene expression/miRNA profiling, SNP genotyping, Copy number variation, Protein thermal shift, High resolution melt and Pathogen detection as described in -https://assets.thermofisher.com/TFS-Assets/LSG/manuals/4489823.pdf

·         Chemistry: The Quantstudio6 and 7900HT have compatible Taqman and SYBR chemistries as shown in- http://assets.thermofisher.com/TFS-Assets/GSD/Flyers/real-time-pcr--master-mixes-instrument-compatibility-flyer.pdf

·         Software:  for instructions on how to setup your experiments click-

https://assets.thermofisher.com/TFS-Assets/LSG/manuals/4489822.pdf

·         Software Download: The windows 7 compatible lisence-free software can be downloaded from -http://www.thermofisher.com/us/en/home/technical-resources/software-downloads/quantstudio-flex-real-time-pcr-system.html

If you have any further questions please don hesitate to contact the MHTP Medical Genomics Laboratory Manager: niro.pathirage@hudson.org.au